Purification of the dimeric form of dextransucrases from Leuconostoc mesenteroides strains NRRL B-1416 and B-1375.
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منابع مشابه
Purification and Characterization of Dextransucrase from Leuconostoc Mesenteroides Nrrl B-1149
Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...
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Article info: Received: 22 February 2012 In revised form: 28 April 2012 Accepted: 30 April 2012 ABSTRACT Dextransucrases are enzymes that transfer the glucosyl moiety from sucrose to other acceptor molecules. In the present study, we have investigated the biosynthesis of dextransucrases by two strains of Leuconostoc mesenteroides isolated from Bulgarian fermented products. Extracellular dextran...
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The Leuconostoc species require sucrose in the culture medium as an inducer for the elaboration of glucosyltransferase (GTF) and glucans. Among all the reported putification methods, polyethylene glycol (PEG) is a effective and single step purification method for GTF from Leuconostoc mesenteroides. The extracellular GTFs from Leuconostoc mesenteroides L.m.28 and mutant Leuconostoc mesenteroides...
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Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, r...
متن کاملUV Mutagenesis of Leuconostoc mesenteroides NRRL B-640 for Generation of a Mutant (B-640M) with Hyper-Producing Dextransucrase Activity
This study reports the strain improvement of Leuconostoc mesenteroides NRRL B-640 by classical mutation technique using ultraviolet radiation to achieve the mutant strain with higher dextransucrase activity. A large number of mutants were generated, cultured and tested for dextransucrase activity. The selected mutant gave considerably higher (46%) dextransucrase activity (0.8 U/mg) as compared ...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1985
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.49.1385